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Salmonella pullorum strain W was serially passaged on the brain heart infusion agar containing acrdine orange(AO) as a concentration of 101) mcg/㎖. S. pullorum AO60 and S. pullorum A0150, which were subcultured 60 and 150 passages on AO media, were examined for permeability harrier function of the cell wall. A060 and AO150 were appeared to be decreased in susceptibility against hydrophobic substances touch as crystal violet, chloramphenicol and rifamycin, which might be resulted from the changes of permeability barrier function of the cell wall. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacterial protein, the protein profiles of AO6O and AO 150 didn´t differ significantly from W, but increased amount of the band of MW 140,000-145,000 was confirmed. And 〔G+C〕 contents of DNA in AO60 and A0150 were decreased than that of W.
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